ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of Volatile Oil of Radix Angelicae Sinensis (VOA) on experimental asthma in rat model based on abnormal immune functions of Treg cells.</p><p><b>METHODS</b>After grouping, the asthmatic rats were developed through injecting OVA and AI(OH)3 for sensitization and then administering OVA aerosol for challenge, and the respiratory functions, asthmatic behaviors, IL-10 levels in bronchoalveolar lavage fluid (BALF) (ELISA) and Foxp3 expression (immunohistochemistry) in lung of asthmatic rats were observed.</p><p><b>RESULTS</b>VOA at the doses of 40-160 mg/kg could improve the respiratory functions and the asthmatic behaviors, and upgrade IL-10 levels in BALF and Foxp3 expression in lung of asthmatic rats.</p><p><b>CONCLUSION</b>VOA has some effects of anti-asthma and one of the mechanisms is to improving the lower immune functions of Treg cells.</p>
Subject(s)
Animals , Rats , Angelica sinensis , Chemistry , Asthma , Drug Therapy , Bronchoalveolar Lavage Fluid , Chemistry , Disease Models, Animal , Forkhead Transcription Factors , Metabolism , Interleukin-10 , Chemistry , Lung , Metabolism , Oils, Volatile , Pharmacology , Plant Oils , Pharmacology , T-Lymphocytes, Regulatory , Cell BiologyABSTRACT
<p><b>OBJECTIVE</b>To investigate the influences of ultrafiltration and alcohol sedimentation on protective effects of Radix Astragali and Radix Hedyseri against rat's cerebral ischemia.</p><p><b>METHODS</b>Using dexamethasone (im.) and ligating common carotid artery, the rat stasis model combined transient cerebral ischemia was established to evaluate the effects of the ultrafiltration and alcohol sedimentation through detecting antioxidant system and other indexes in brain tissue.</p><p><b>RESULTS</b>The results showed that the 6 g/kg water extract(crude drug), ultrafiltration and alcohol sedimentation of Radix Astragali and Radix Hedyseri could upgrade adenosine-triphosphate (ATP), superoxide dismutase (SOD) and catalase (CAT), and degrade malondialdehyde(MDA) and water content of brain tissue in rat stasis model combined transient cerebral ischemia, the water extract and ultrafiltration of them could degrade lactic acid (LD) of brain tissue, and the effects of alcohol sedimentation of Radix Astragali and Radix Hedyseri become weaker than water extract of them.</p><p><b>CONCLUSION</b>The water extract, ultrafiltration and alcohol sedimentation of Radix Astragali and Radix Hedyseri have some protective effects on cerebral ischemia in rats, the effective differences of the extract through the same extraction method are not remarkable, and alcohol precipitation method has obvious influences effect on Radix Astragali and Radix Hedyseri.</p>
Subject(s)
Animals , Rats , Alcohols , Chemistry , Antioxidants , Metabolism , Astragalus Plant , Chemistry , Brain , Catalase , Metabolism , Cerebral Infarction , Drug Therapy , Drugs, Chinese Herbal , Pharmacology , Malondialdehyde , Metabolism , Plant Roots , Chemistry , Superoxide Dismutase , Metabolism , UltrafiltrationABSTRACT
<p><b>OBJECTIVE</b>To comparatively study the effects of Rhubarbs from different regions on blood lipid and antioxi dation of hyperlipidemia rats.</p><p><b>METHODS</b>Male rats were randomly divided into 9 groups ( n = 8) and fed with high-fat diet to replicate the hyperlipidemia model. Meanwhile, Rheum tanguticum was administrated intragastrically at two doses (3.0 g/kg and 1.0 g/kg), once a day for continuous 28 days. The effects of Rheum tanguticum planted in Gannan (RT-GN), Rheum tanguticum planted in Xinin (RT-XN) and Rheum plmatum planted in Lixian (RP-LX) were evaluated through detecting the parameters of blood lipids, blood viscosity and antioxidant system.</p><p><b>RESULTS</b>T-GN, RT-XN and RP-LX in the range of 1.0-3.0 g/kg could decrease the blood levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) and malonaldehyde (MDA) in blood. Besides, they could reduce blood viscosity, increase high density lipoprotein (HDL) level and upregulate the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Interestingly, their effects on blood viscosity was obviously in a dose dependent manner. In addition, the effects of RT-GN on LDL, MDA and blood viscosity were not significantly different from those of RT-XN and better than those of RP-LX.</p><p><b>CONCLUSION</b>The RT has better hypolipidemic effects than the RP, but RT-GN and RT-XN are not different from the above effects.</p>
Subject(s)
Animals , Male , Rats , Antioxidants , Metabolism , Blood Viscosity , Cholesterol , Blood , Diet, High-Fat , Disease Models, Animal , Drugs, Chinese Herbal , Pharmacology , Glutathione Peroxidase , Metabolism , Hyperlipidemias , Drug Therapy , Lipids , Blood , Lipoproteins, HDL , Blood , Lipoproteins, LDL , Blood , Malondialdehyde , Blood , Rheum , Chemistry , Superoxide Dismutase , Metabolism , Triglycerides , BloodABSTRACT
<p><b>OBJECTIVE</b>To study the effects of JTS, Traditional Chinese Medicine caps. treating cerebral ischemia on metabolism and antioxidant system in cerebral ischemia rat.</p><p><b>METHODS</b>I.m. dexamethasone and ligating common carotid artery, the model of cerebral ischemia rats was established to investigate the effects of JTS caps. and its mechanisms through detecting substance metabolism, energy metabolism and antioxidant system.</p><p><b>RESULTS</b>JTS caps. (1.78 - 3.56 g/kg) could upgrade glucose (Glu), total amino acids (T-AA), ATP, Na(+)-K(+) -ATPase, superoxide dismutase (SOD) and catalase (CAT) of brain tissue and degrade lactic acid (LD), malondialdehyde (MDA) and water content of brain tissue in cerebral ischemia rat (P < 0.05, 0.01). JTS caps. (3.56 g/kg) could also depress extenuation of rat's body weight.</p><p><b>CONCLUSION</b>JTS caps. has some protections against the cerebral ischemia in rats, and one of the mechanisms may be improving the metabolism and antioxidant system.</p>